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elution

C2
noun

Pronunciation

UK

  • /ɪlˈuːʃən/

US

  • /ɪlˈuːʃən/

Description

Imagine you're making tea. Hot water passes through the leaves and carries the flavor and color away with it. In science, elution works in a similar way: a liquid, and sometimes a gas, moves through a material and carries a substance out of it. This is often used in lab work to separate and collect different parts of a mixture.

You will most often see this in chemistry, biology, and especially chromatography, a method for testing or purifying substances. If a scientist says they are doing an "elution step," they mean they are washing a wanted substance off a material so it can be collected.

Examples

  1. 1

    DNA extraction

    The DNA was collected during elution, after the washing steps were finished.

  2. 2

    Chromatography method

    The lab switched to gradient elution to separate the two compounds more clearly.

    • Domain

      gradient elution

      elution where the liquid conditions change step by step

  3. 3

    Troubleshooting

    Poor elution left part of the sample in the column, so the team adjusted the solvent.

Forms and spellings

1 form open this card.

Main spelling

  • elutionnoun